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Blood, 26 February 2009, Vol. 113, No. 9, pp. 2079-2087. Prepublished online as a Blood First Edition Paper on January 8, 2009; DOI 10.1182/blood-2008-09-177857. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2008-09-177857v1 113/9/2079    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kelher, M. R. Articles by Silliman, C. C. Search for Related Content PubMed PubMed Citation Articles by Kelher, M. R. Articles by Silliman, C. C. Related Collections Transfusion Medicine Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  TRANSFUSION MEDICINE Plasma from stored packed red blood cells and MHC class I antibodies causes acute lung injury in a 2-event in vivo rat model Marguerite R. Kelher1,2, Tomhiko Masuno2,3, Ernest E. Moore2,4, Sagar Damle2,4, Xianzhong Meng2, Yong Song2, Xiayuan Liang5, Jerry Niedzinski6, Steven S. Geier6, Samina Y. Khan1,2,7, Fabia Gamboni-Robertson2, and Christopher C. Silliman1,2,7 1 Bonfils Blood Center, Denver, CO; 2 Department of Surgery, School of Medicine, University of Colorado Denver, Aurora; 3 Department of Emergency and Critical Care Medicine, Nippon Medical School, Tokyo, Japan; 4 Department of Surgery, Denver Health Medical Center, CO; 5 Department of Pathology, The Children's Hospital, Aurora, CO; 6 Human Tissue Typing Laboratory, LABS, Centennial, CO; and 7 Department of Pediatrics, School of Medicine, University of Colorado Denver, Aurora Transfusion-related acute lung injury (TRALI) is the leading cause of transfusion death. We hypothesize that TRALI requires 2 events: (1) the clinical condition of the patient and (2) the infusion of antibodies against MHC class I antigens or the plasma from stored blood. A 2-event rat model was developed with saline (NS) or endotoxin (LPS) as the first event and the infusion of plasma from packed red blood cells (PRBCs) or antibodies (OX18 and OX27) against MHC class I antigens as the second event. ALI was determined by Evans blue dye leak from the plasma to the bronchoalveolar lavage fluid (BALF), protein and CINC-1 concentrations in the BALF, and the lung histology. NS-treated rats did not evidence ALI with any second events, and LPS did not cause ALI. LPS-treated animals demonstrated ALI in response to plasma from stored PRBCs, both prestorage leukoreduced and unmodified, and to OX18 and OX27, all in a concentration-dependent fashion. ALI was neutrophil (PMN) dependent, and OX18/OX27 localized to the PMN surface in vivo and primed the oxidase of rat PMNs. We conclude that TRALI is the result of 2 events with the second events consisting of the plasma from stored blood and antibodies that prime PMNs. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

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