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Blood, 2 July 2009, Vol. 114, No. 1, pp. 33-37. Prepublished online as a Blood First Edition Paper on May 6, 2009; DOI 10.1182/blood-2009-01-197368. This Article Full Text Full Text (PDF) Supplemental Appendix All Versions of this Article: blood-2009-01-197368v1 114/1/33    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Nieto, W. G. Articles by Orfao, A. Search for Related Content PubMed PubMed Citation Articles by Nieto, W. G. Articles by Orfao, A. Related Collections Free Research Articles Lymphoid Neoplasia Brief Reports Clinical Trials and Observations Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  CLINICAL TRIALS AND OBSERVATIONS Brief report Increased frequency (12%) of circulating chronic lymphocytic leukemia–like B-cell clones in healthy subjects using a highly sensitive multicolor flow cytometry approach Wendy G. Nieto1, Julia Almeida1, Alfonso Romero2, Cristina Teodosio1, Antonio López1, Ana F. Henriques1, Maria Luz Sánchez1, María Jara-Acevedo1, Ana Rasillo1, Marcos González3, Paulino Fernández-Navarro4, Tomás Vega5, Alberto Orfao1, and the Primary Health Care Group of Salamanca for the Study of MBL5 1 Instituto de Biología Molecular y Celular del Cáncer, Centro de Investigación del Cáncer/IBMCC (CSIC-USAL) & Cytometry Service and Department of Medicine, University of Salamanca, Salamanca; 2 Gerencia de Atención Primaria de Salud de Salamanca, Sanidad de Castilla y León (SACYL), Castilla y León; 3 Servicio de Hematología, Hospital Universitario de Salamanca, Salamanca; 4 Centro de Atención Primaria de Salud de Ledesma Salamanca, Sanidad de Castilla y León (SACYL), Castilla y León; and 5 Dirección General de Salud Pública y Consumo, Junta de Castilla y León, Castilla y León, Spain Monoclonal B-cell lymphocytosis (MBL) indicates the presence of less than 5 x 109/L circulating monoclonal B cells in otherwise healthy subjects. Recently, it has been reported that circulating chronic lymphocytic leukemia (CLL)–like B cells can be detected using 4- or 5-multicolor flow cytometry in 5% to 7% of adults with normal lymphocyte counts. We investigated the frequency of circulating monoclonal B cells in 608 healthy subjects older than 40 years with normal blood counts, using a highly sensitive 8-color flow cytometry approach and systematic screening for total PB leukocyte count higher than 5 x 106. We show that the frequency of PB monoclonal B cells is markedly higher than previously reported (12% for CLL-like B cells, found at frequencies of 0.17 ± 0.13 x 109 cells/L), the incidence progressively increasing with age. Most cases (62%) showed clonal B-cell levels below the maximum sensitivity of the techniques described by others (< 0.01%), supporting the notion that detection of MBL may largely depend on the sensitivity of the flow cytometry approach used. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. D. Boyd, E. L. Marshall, J. D. Merker, J. M. Maniar, L. N. Zhang, B. Sahaf, C. D. Jones, B. B. Simen, B. Hanczaruk, K. D. Nguyen, et al. Measurement and Clinical Monitoring of Human Lymphocyte Clonality by Massively Parallel V-D-J Pyrosequencing Science Translational Medicine, December 23, 2009; 1(12): 12ra23 - 12ra23. [Abstract] [Full Text] [PDF] A. C. Rawstron Monoclonal B-cell lymphocytosis Hematology, January 1, 2009; 2009(1): 430 - 439. [Abstract] [Full Text] [PDF]

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