Blood online
Home About Blood Authors Subscriptions Permission Advertising Public Access contact us
 

 
Advanced
Current Issue
First Edition
Future Articles
Archives
Submit to Blood
Search
American Society of Hematology
Meeting Abstracts
Email Alerts
 Blood, 2 July 2009, Vol. 114, No. 1, pp. 10-19.
Prepublished online as a Blood First Edition Paper on May 12, 2009; DOI 10.1182/blood-2009-02-203828.

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Methods and Tables
Right arrow All Versions of this Article:
blood-2009-02-203828v1
114/1/e10    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Right arrow Rights and Permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Google Scholar
Right arrow Articles by Lewandrowski, U.
Right arrow Articles by Sickmann, A.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lewandrowski, U.
Right arrow Articles by Sickmann, A.
Related Collections
Right arrow Platelets and Thrombopoiesis
Right arrow e-Blood
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

arrow to previous article Previous Article  |  Table of Contents

e-Blood

Platelet membrane proteomics: a novel repository for functional research

Urs Lewandrowski1, Stefanie Wortelkamp1, Katharina Lohrig2, René P. Zahedi1, Dirk A. Wolters2, Ulrich Walter3, and Albert Sickmann1,4

1 Institute for Analytical Sciences (ISAS), Dortmund; 2 Department of Analytical Chemistry, Ruhr-University-Bochum, Bochum; 3 Institute for Clinical Biochemistry and Pathobiochemistry and Central Laboratory, University Würzburg, Würzburg; and 4 Medizinisches Proteom-Center (MPC), Ruhr-University-Bochum, Bochum, Germany

Being central players in thrombosis and hemostasis, platelets react in manifold and complex ways to extracellular stimuli. Cell-matrix and cell-cell interactions are mandatory for initial adhesion as well as for final development of stable plugs. Primary interfaces for interactions are plasma membrane proteins, of which many have been identified over the past decades in individual studies. However, due to their enucleate structure, platelets are not accessible to large-scale genomic screens and thus a comprehensive inventory of membrane proteins is still missing. For this reason, we here present an advanced proteomic setup for the detailed analysis of enriched platelet plasma membranes and the so far most complete collection of platelet membrane proteins. In summary, 1282 proteins were identified, of which more than half are termed to be of membrane origin. This study provides a brief overview of gene ontology subcellular and functional classification, as well as interaction network analysis. In addition, the mass spectrometric data were used to assemble a first tentative relative quantification of large-scale data on the protein level. We therefore estimate the presented data to be of major interest to the platelet research field and to support rational design of functional studies.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




click for free articles
home about blood authors subscriptions permissions advertising public access contact us
  Copyright © 2009 by American Society of Hematology         Online ISSN: 1528-0020