SEARCH:   Advanced

Blood, 10 September 2009, Vol. 114, No. 11, pp. 2323-2332. Prepublished online as a Blood First Edition Paper on July 17, 2009; DOI 10.1182/blood-2008-10-183814. This Article Full Text Full Text (PDF) Supplemental Figures All Versions of this Article: blood-2008-10-183814v1 114/11/2323    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Google Scholar Articles by Pfistershammer, K. Articles by Steinberger, P. PubMed PubMed Citation Articles by Pfistershammer, K. Articles by Steinberger, P. Related Collections Transplantation Free Research Articles Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  TRANSPLANTATION Allogeneic disparities in immunoglobulin-like transcript 5 induce potent antibody responses in hematopoietic stem cell transplant recipients Katharina Pfistershammer1, Anita Lawitschka2, Christoph Klauser3, Judith Leitner3, Roman Weigl4, Mirjam H. M. Heemskerk5, Winfried F. Pickl3, Otto Majdic3, Georg A. Böhmig6, Gottfried F. Fischer7, Hildegard T. Greinix4, and Peter Steinberger3 1 Department of Dermatology, Division of Immunology, Allergy and Infectious Diseases, Medical University of Vienna, Vienna, Austria; 2 St Anna Children's Hospital, Vienna, Austria; 3 Institute of Immunology, Center for Physiology, Pathophysiology and Immunology, and 4 Department of Internal Medicine I, Bone Marrow Transplantation Unit, Medical University of Vienna, Vienna, Austria; 5 Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands; and 6 Division of Nephrology and Dialysis, Department of Medicine III, and 7 Department of Blood Group Serology, Medical University of Vienna, Vienna, Austria In hematopoietic stem cell transplant (HSCT) recipients, the recognition of polymorphic antigens by the donor-derived immune system is an important mechanism underlying both graft-versus-host disease and graft-versus-leukemia (GVL) effect. Here we show that a subset of HSCT recipients (13.9%, n = 108) have antibodies directed to surface molecules of dendritic cells. We have used one such serum in conjunction with retroviral expression cloning to identify the highly polymorphic surface molecule immunoglobulin-like transcript 5 (ILT5) as one of the targets of dendritic cell-reactive antibodies. ILT5 reactive antibodies were found in 5.4% of HSCT patients but not in solid organ transplantation recipients, patients with collagen diseases, multiparous women, or polytransfused or healthy persons. We show that ILT5-specific antibodies can mediate killing of ILT5-bearing cells and furthermore demonstrate ILT5 expression in some leukemic cells, indicating that it might be a target for GVL effects. Thus, our results represent the first description of potent allogeneic antibody responses to a non–major histocompatibility complex cell surface molecule in hematopoietic stem cell transplanted patients and warrant further studies to elucidate the role of antibodies to polymorphic cell surface molecules in GVL and graft-versus-host responses. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

	Copyright © 2009 by American Society of Hematology         Online ISSN: 1528-0020