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Blood, 1 October 2009, Vol. 114, No. 14, pp. 3127-3130. Prepublished online as a Blood First Edition Paper on July 23, 2009; DOI 10.1182/blood-2008-12-190991. This Article Full Text Full Text (PDF) All Versions of this Article: blood-2008-12-190991v1 114/14/3127    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Google Scholar Articles by Piaggio, G. Articles by Barosi, G. PubMed PubMed Citation Articles by Piaggio, G. Articles by Barosi, G. Related Collections Vascular Biology Brief Reports Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  VASCULAR BIOLOGY Brief report Endothelial colony-forming cells from patients with chronic myeloproliferative disorders lack the disease-specific molecular clonality marker Giovanna Piaggio1,*, Vittorio Rosti2,*, Mirko Corselli3, Francesca Bertolotti3, Gaetano Bergamaschi4, Sarah Pozzi3, Davide Imperiale3, Barbara Chiavarina3, Elisa Bonetti5, Francesca Novara6, Mario Sessarego7, Laura Villani5, Anna Garuti8, Margherita Massa9, Riccardo Ghio10, Rita Campanelli5, Andrea Bacigalupo1, Alessandro Pecci11, Gianluca Viarengo12, Orsetta Zuffardi6, Francesco Frassoni3,, and Giovanni Barosi5, 1 Divisione Ematologia, Ospedale San Martino, Genova; 2 Laboratory of Organ Transplantation, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Policlinico San Matteo Foundation, Pavia; 3 Centro Cellule Staminali e Terapia Cellulare, Ospedale San Martino, Genova; 4 Medicina Interna ed Oncologia Medica, IRCCS Fondazione Policlinico San Matteo, Pavia; 5 Unit of Clinical Epidemiology-Center for the Study of Myelofibrosis, IRCCS Policlinico San Matteo Foundation, Pavia; 6 Dipartimento Genetica Umana, University of Pavia, Pavia; 7 Laboratorio Diagnostica Emato-Oncologica, Biomolecolare e Citogenetica, Dipartimento Medicina Intema, Università di Genova, Genova; 8 Laboratorio Terapie Cellulari, Dipartimento Medicina Intema, Università di Genova, Genoa; 9 Laboratory of Biotechnology, IRCCS Policlinico San Matteo Foundation, Pavia; 10 Clinica di Medicina Interna 3, Ospedale San Martino, Genoa; 11 Dipartimento di Medicina Interna, Universita'di Pavia IRCCS Fondazione Policlinico San Matteo, Pavia; and 12 Immunoemathology and Transfusion Service, IRCCS Policlinico San Matteo Foundation, Pavia, Italy Two putative types of circulating endothelial progenitor cells have been recently identified in vitro: (1) endothelial colony-forming cell (ECFC) and (2) colony-forming unit–endothelial cell (CFU-EC). Only the former is now recognized to belong to endothelial lineage. We have used the ECFC and CFU-EC assays to readdress the issue of the clonal relation between endothelial progenitor cells and hematopoietic stem cells in patients with Philadelphia-positive and Philadelphia-negative chronic myeloproliferative disorders. Both ECFCs and CFU-ECs were cultured from peripheral blood mononuclear cells, and either BCR-ABL rearrangement or JAK2-V617F mutation were assessed in both types of endothelial colonies. We found that ECFCs lack the disease-specific markers, which are otherwise present in CFU-ECs, thus reinforcing the concept that the latter belongs to the hematopoietic lineage, and showing that in chronic myeloproliferative disorders the cell that gives rise to circulating ECFC has a distinct origin from the cell of the hematopoietic malignant clone. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

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