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Blood, 8 October 2009, Vol. 114, No. 15, pp. 3227-3234. Prepublished online as a Blood First Edition Paper on August 10, 2009; DOI 10.1182/blood-2009-06-228759. This Article Full Text Full Text (PDF) Supplemental Figures and Videos All Versions of this Article: blood-2009-06-228759v1 114/15/3227    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Google Scholar Articles by Beuneu, H. Articles by Bousso, P. PubMed PubMed Citation Articles by Beuneu, H. Articles by Bousso, P. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Dynamic behavior of NK cells during activation in lymph nodes Hélène Beuneu1,2, Jacques Deguine1,2, Béatrice Breart1,2, Ofer Mandelboim3, James P. Di Santo4, and Philippe Bousso1,2 1 Institut Pasteur, G5 Dynamiques des Réponses Immunes, Paris, France; 2 Inserm U668, Equipe Avenir, Paris, France; 3 The Lautenberg Center for General and Tumor Immunology, The Hebrew University-Hadassah Medical School, Jerusalem, Israel; and 4 Institut Pasteur, Unité des Cytokines et Développement Lymphoïde; Inserm U668, Paris, France During infection, Toll-like receptor agonists induce natural killer (NK)–cell activation by stimulating dendritic cells (DCs) to produce cytokines and transpresent IL-15 to NK cells. Yet the cellular dynamics underlying NK-cell activation by DCs in secondary lymphoid organs are largely unknown. Here, we have visualized NK-cell activation using mice in which NK cells and DCs express different fluorescent proteins. In response to polyI:C or lipopolysaccharide, NK cells maintained a vigorous migratory behavior, establishing multiple short contacts with maturing DCs. Furthermore, mature antigen-loaded DCs that made long-lived interactions with T cells formed short-lived contacts with NK cells. The different behaviors of T cells and NK cells during activation was correlated with distinct calcium responses upon interaction with DCs. That NK cells become activated while remaining motile may constitute an efficient strategy for sampling local concentrations of cytokines around DCs in secondary lymphoid tissues. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

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