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Blood, 22 October 2009, Vol. 114, No. 17, pp. 3578-3587. Prepublished online as a Blood First Edition Paper on August 21, 2009; DOI 10.1182/blood-2009-03-213561. This Article Full Text Full Text (PDF) Supplemental Methods and Figures All Versions of this Article: blood-2009-03-213561v1 114/17/3578    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Google Scholar Articles by Dutta, P. Articles by Burlingham, W. J. PubMed PubMed Citation Articles by Dutta, P. Articles by Burlingham, W. J. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Microchimerism is strongly correlated with tolerance to noninherited maternal antigens in mice Partha Dutta1,2, Melanie Molitor-Dart1, Joseph L. Bobadilla1, Drew A. Roenneburg1, Zhen Yan3, Jose R. Torrealba4, and William J. Burlingham1 1 Department of Surgery, University of Wisconsin, Madison; 2 Departments of Pathobiological Sciences and Comparative Biological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison; 3 Department of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, WA; and 4 Department of Pathology, University of Wisconsin, Madison In mice and humans, the immunologic effects of developmental exposure to noninherited maternal antigens (NIMAs) are quite variable. This heterogeneity likely reflects differences in the relative levels of NIMA-specific T regulatory (TR) versus T effector (TE) cells. We hypothesized that maintenance of NIMA-specific TR cells in the adult requires continuous exposure to maternal cells and antigens (eg, maternal microchimerism [MMc]). To test this idea, we used 2 sensitive quantitative polymerase chain reaction (qPCR) tests to detect MMc in different organs of NIMAd-exposed H2b mice. MMc was detected in 100% of neonates and a majority (61%) of adults; nursing by a NIMA+ mother was essential for preserving MMc into adulthood. MMc was most prevalent in heart, lungs, liver, and blood, but was rarely detected in unfractionated lymphoid tissues. However, MMc was detectable in isolated CD4+, CD11b+, and CD11c+ cell subsets of spleen, and in lineage-positive cells in heart. Suppression of delayed type hypersensitivity (DTH) and in vivo lymphoproliferation correlated with MMc levels, suggesting a link between TR and maternal cell engraftment. In the absence of neonatal exposure to NIMA via breastfeeding, MMc was lost, which was accompanied by sensitization to NIMA in some offspring, indicating a role of oral exposure in maintaining a favorable TR > TE balance. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. G. S. van Halteren, E. Jankowska-Gan, A. Joosten, E. Blokland, J. Pool, A. Brand, W. J. Burlingham, and E. Goulmy Naturally acquired tolerance and sensitization to minor histocompatibility antigens in healthy family members Blood, September 10, 2009; 114(11): 2263 - 2272. [Abstract] [Full Text] [PDF]

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