Blood online
Home About Blood Authors Subscriptions Permission Advertising Public Access contact us
 

 
Advanced
Current Issue
First Edition
Future Articles
Archives
Submit to Blood
Search
American Society of Hematology
Meeting Abstracts
Email Alerts
 Blood, 5 November 2009, Vol. 114, No. 19, pp. 4310-4319.
Prepublished online as a Blood First Edition Paper on August 28, 2009; DOI 10.1182/blood-2009-03-211342.

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Methods, Figures, and Videos
Right arrow All Versions of this Article:
blood-2009-03-211342v1
114/19/4310    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Right arrow Rights and Permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Google Scholar
Right arrow Articles by Madlambayan, G. J.
Right arrow Articles by Cogle, C. R.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Madlambayan, G. J.
Right arrow Articles by Cogle, C. R.
Related Collections
Right arrow Vascular Biology
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

arrow to previous article Previous Article  |  Table of Contents  |  Next Article next article arrow

VASCULAR BIOLOGY

Bone marrow stem and progenitor cell contribution to neovasculogenesis is dependent on model system with SDF-1 as a permissive trigger

Gerard J. Madlambayan*,1, Jason M. Butler*,1, Koji Hosaka1, Marda Jorgensen1, Dongtao Fu1, Steven M. Guthrie1, Anitha K. Shenoy1, Adam Brank1, Kathryn J. Russell1, Jaclyn Otero1, Dietmar W. Siemann2, Edward W. Scott1, and Christopher R. Cogle1

1 Program in Stem Cell Biology and Regenerative Medicine, and 2 Department of Radiation Oncology and Shands Cancer Center, University of Florida, Gainesville

Adult bone marrow (BM) contributes to neovascularization in some but not all settings, and reasons for these discordant results have remained unexplored. We conducted novel comparative studies in which multiple neovascularization models were established in single mice to reduce variations in experimental methodology. In different combinations, BM contribution was detected in ischemic retinas and, to a lesser extent, Lewis lung carcinoma cells, whereas B16 melanomas showed little to no BM contribution. Using this spectrum of BM contribution, we demonstrate the necessity for site-specific expression of stromal-derived factor-1{alpha} (SDF-1{alpha}) and its mobilizing effects on BM. Blocking SDF-1{alpha} activity with neutralizing antibodies abrogated BM-derived neovascularization in lung cancer and retinopathy. Furthermore, secondary transplantation of single hematopoietic stem cells (HSCs) showed that HSCs are a long-term source of neovasculogenesis and that CD133+CXCR4+ myeloid progenitor cells directly participate in new blood vessel formation in response to SDF-1{alpha}. The varied BM contribution seen in different model systems is suggestive of redundant mechanisms governing postnatal neovasculogenesis and provides an explanation for contradictory results observed in the field.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




click for free articles
home about blood authors subscriptions permissions advertising public access contact us
  Copyright © 2009 by American Society of Hematology         Online ISSN: 1528-0020