SEARCH:   Advanced

Blood, 9 July 2009, Vol. 114, No. 2, pp. 328-337. Prepublished online as a Blood First Edition Paper on May 5, 2009; DOI 10.1182/blood-2008-12-192203. This Article Full Text Full Text (PDF) Supplemental Methods and Figures All Versions of this Article: blood-2008-12-192203v1 114/2/328    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Google Scholar Articles by Di Mascio, M. Articles by Lane, H. C. PubMed PubMed Citation Articles by Di Mascio, M. Articles by Lane, H. C. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Noninvasive in vivo imaging of CD4 cells in simian-human immunodeficiency virus (SHIV)–infected nonhuman primates Michele Di Mascio1, Chang H. Paik2, Jorge A. Carrasquillo3, Jin-Soo Maeng2, Beom-Su Jang2, In Soo Shin2, Sharat Srinivasula4, Russ Byrum5, Achilles Neria2, William Kopp6, Marta Catalfamo1, Yoshiaki Nishimura1, Keith Reimann7, Malcolm Martin1, and H. Clifford Lane1 1 National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH), Bethesda, MD; 2 Warren G. Magnuson Clinical Center, NIH, Bethesda, MD; 3 Memorial Sloan-Kettering Cancer Center, New York, NY; 4 Biostatistics Research Branch, SAIC-Frederick Inc, National Cancer Institute (NCI)–Frederick, MD; 5 Bioqual, Rockville, MD; 6 Clinical Services Program, NCI-Frederick, MD; and 7 Beth Israel Deaconess Medical Center, Boston, MA Since the earliest days of the HIV epidemic, the number of CD4+ T cells per unit volume of blood has been recognized as a major prognostic factor for the development of AIDS in persons with HIV infection. It has also been generally accepted that approximately 2% of total body lymphocytes circulate in the blood. In the present study, we have used a nondepleting humanized anti-CD4 monoclonal antibody labeled with the gamma emitter indium-111 to visualize the CD4+ T-cell pool in vivo in nonhuman primates with simian HIV infection. A strong correlation was noted between radiotracer uptake in spleen, tonsil, axillary lymph nodes, and peripheral blood CD4 T-cell counts ( = 0.75, 0.93, and 0.85, respectively, P < .005). The relationship between radiotracer retention in lymphoid tissues and CD4+ T-cell counts in the circulation was governed by an exponential law. These data provide an estimate for the total number of lymphocytes in the body as being between 1.9 and 2.9 x 1012 and suggest that the partition between peripheral blood and lymphoid tissue is between 0.3% and 0.5%. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

	Copyright © 2009 by American Society of Hematology         Online ISSN: 1528-0020