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Blood, 19 November 2009, Vol. 114, No. 21, pp. 4664-4674. Prepublished online as a Blood First Edition Paper on September 2, 2009; DOI 10.1182/blood-2009-05-221598. This Article Full Text Full Text (PDF) Supplemental Methods and Figures Additional Supplemental Figures All Versions of this Article: blood-2009-05-221598v1 114/21/4664    most recent e-Letter: Submit a Response Alert me when this article is cited Alert me when e-Letters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Van den Bossche, J. Articles by Van Ginderachter, J. A. Search for Related Content PubMed PubMed Citation Articles by Van den Bossche, J. Articles by Van Ginderachter, J. A. Related Collections Immunobiology Phagocytes, Granulocytes, and Myelopoiesis Related Article in Blood Online Social Bookmarking                         What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Alternatively activated macrophages engage in homotypic and heterotypic interactions through IL-4 and polyamine-induced E-cadherin/catenin complexes Jan Van den Bossche1,2, Pieter Bogaert3, Jolanda van Hengel4,5, Christopher J. Guérin4,6, Geert Berx4,7, Kiavash Movahedi1,2, Rafael Van den Bergh1,2, Anna Pereira-Fernandes1,2, Jan M. C. Geuns8, Hanspeter Pircher9, Pierre Dorny10, Johan Grooten3, Patrick De Baetselier1,2, and Jo A. Van Ginderachter1,2 1 Department of Molecular and Cellular Interactions, VIB and 2 Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium; 3 Laboratory of Molecular Immunology, Department of Molecular Biomedical Research and 4 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium; 5 Molecular Cell Biology Unit, 6 Microscopy Core Facility, and 7 Unit of Molecular and Cellular Oncology, Department for Molecular Biomedical Research, VIB, Ghent, Belgium; 8 Laboratory of Functional Biology, KU Leuven, Heverlee, Belgium; 9 Department of Immunology, Institute of Medical Microbiology and Hygiene, University of Freiburg, Freiburg, Germany; and 10 Department of Animal Health, Institute of Tropical Medicine, Antwerp, Belgium Alternatively activated macrophages (AAMs), triggered by interleukin-4 (IL-4) and IL-13, play a modulating role during Th2 cytokine-driven pathologies, but their molecular armament remains poorly characterized. Here, we established E-cadherin (Cdh1) as a selective marker for IL-4/IL-13–exposed mouse and human macrophages, which is STAT6-dependently induced during polarized Th2 responses associated with Taenia crassiceps helminth infections or allergic airway inflammation. The IL-4–dependent, arginase-1/ornithine decarboxylase–mediated production of polyamines is important for maximal Cdh1 induction, unveiling a novel mechanism for IL-4–dependent gene transcription. At the macrophage surface, E-cadherin forms a functional complex with the catenins that accumulates at sites of cell contact. Macrophage-specific deletion of the Cdh1 gene illustrates the implication of E-cadherin in IL-4–driven macrophage fusion and heterotypic interactions with CD103+ and KLRG1+ T cells. This study identifies the E-cadherin/catenin complex as a discriminative, partly polyamine-regulated feature of IL-4/IL-13–exposed alternatively activated macrophages that contributes to homotypic and heterotypic cellular interactions. CiteULike    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this? Related Article in Blood Online: Macrophage fusion cuisine Antonio Sica and Alberto Mantovani Blood 2009 114: 4609-4610. [Full Text] [PDF] This article has been cited by other articles: A. Sica and A. Mantovani Macrophage fusion cuisine Blood, November 19, 2009; 114(21): 4609 - 4610. [Full Text] [PDF]

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