SEARCH:   Advanced

Blood, 23 July 2009, Vol. 114, No. 4, pp. 826-834. Prepublished online as a Blood First Edition Paper on May 26, 2009; DOI 10.1182/blood-2009-01-198580. This Article Full Text Full Text (PDF) Supplemental Methods, Tables, and Figures All Versions of this Article: blood-2009-01-198580v1 114/4/826    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Google Scholar Articles by Leich, E. Articles by Rosenwald, A. PubMed PubMed Citation Articles by Leich, E. Articles by Rosenwald, A. Related Collections Lymphoid Neoplasia Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  LYMPHOID NEOPLASIA Follicular lymphomas with and without translocation t(14;18) differ in gene expression profiles and genetic alterations Ellen Leich1,*, Itziar Salaverria2,*, Silvia Bea2, Andreas Zettl1, George Wright3, Victor Moreno4, Randy D. Gascoyne5, Wing-Chung Chan6, Rita M. Braziel7, Lisa M. Rimsza8, Dennis D. Weisenburger6, Jan Delabie9, Elaine S. Jaffe10, Andrew Lister11, Jude Fitzgibbon11, Louis M. Staudt12, Elena M. Hartmann1, Hans-Konrad Mueller-Hermelink1, Elias Campo2,, German Ott1,13,, and Andreas Rosenwald1, 1 Institute of Pathology, University of Wuerzburg, Wuerzburg, Germany; 2 Department of Pathology, University of Barcelona, Hospital Clinic, Barcelona, Spain; 3 Biometric Research Branch, National Cancer Institute (NCI), Rockville, MD; 4 Biostatistics and Bioinformatics Unit, IDIBELL-Catalan Institute of Oncology, University of Barcelona, Barcelona, Spain; 5 British Columbia Cancer Agency, University of British Columbia, Vancouver, BC; 6 Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha; 7 Southwest Oncology Group, Oregon Health & Science University, Portland; 8 Department of Pathology, University of Arizona, Tucson; 9 Division of Pathology, Norwegian Radium Hospital, Oslo, Norway; 10 Laboratory of Pathology, NCI, Bethesda, MD; 11 Cancer Research UK, St Bartholomew's Hospital, London, United Kingdom; 12 Metabolism Branch, NCI, Bethesda, MD; and 13 Institute of Clinical Pathology, Robert-Bosch-Krankenhaus and Institute of Clinical Pharmacology, Stuttgart, Germany Follicular lymphoma (FL) is genetically characterized by the presence of the t(14;18)(q32;q21) chromosomal translocation in approximately 90% of cases. In contrast to FL carrying the t(14;18), their t(14;18)-negative counterparts are less well studied about their immunohistochemical, genetic, molecular, and clinical features. Within a previously published series of 184 FLs grades 1 to 3A with available gene expression data, we identified 17 FLs lacking the t(14;18). Comparative genomic hybridization and high-resolution single nucleotide polymorphism (SNP) array profiling showed that gains/amplifications of the BCL2 gene locus in 18q were restricted to the t(14;18)-positive FL subgroup. A comparison of gene expression profiles showed an enrichment of germinal center B cell–associated signatures in t(14;18)-positive FL, whereas activated B cell–like, NFB, proliferation, and bystander cell signatures were enriched in t(14;18)-negative FL. These findings were confirmed by immunohistochemistry in an independent validation series of 84 FLs, in which 32% of t(14;18)-negative FLs showed weak or absent CD10 expression and 91% an increased Ki67 proliferation rate. Although overall survival did not differ between FL with and without t(14;18), our findings suggest distinct molecular features of t(14;18)-negative FL. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

	Copyright © 2009 by American Society of Hematology         Online ISSN: 1528-0020