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Blood, 20 August 2009, Vol. 114, No. 8, pp. 1506-1517. Prepublished online as a Blood First Edition Paper on May 28, 2009; DOI 10.1182/blood-2008-09-178863. This Article Full Text Full Text (PDF) Supplemental Table and Figures All Versions of this Article: blood-2008-09-178863v1 114/8/1506    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Google Scholar Articles by Klimchenko, O. Articles by Debili, N. PubMed PubMed Citation Articles by Klimchenko, O. Articles by Debili, N. Related Collections Hematopoiesis and Stem Cells Platelets and Thrombopoiesis Red Cells, Iron, and Erythropoiesis Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS AND STEM CELLS A common bipotent progenitor generates the erythroid and megakaryocyte lineages in embryonic stem cell–derived primitive hematopoiesis Olena Klimchenko13,*, Marcella Mori13,*, Antonio DiStefano13, Thierry Langlois13, Frédéric Larbret13, Yann Lecluse2,3, Olivier Feraud2,4, William Vainchenker13, Françoise Norol13,5,, and Najet Debili13, 1 Inserm U790, Institut Gustave Roussy, Villejuif; 2 Université Paris XI, Institut Gustave Roussy, Villejuif; 3 Institut Gustave Roussy, Villejuif; 4 Plateforme Cellules Souches, Inserm U935, Hôpital Paul Brousse, Villejuif; and 5 Assistance Publique-Hopitaux de Paris, Laboratoire de Thérapie Cellulaire, Hôpital de la Pitié, Paris, France The megakaryocytic (MK) and erythroid lineages are tightly associated during differentiation and are generated from a bipotent megakaryocyte-erythroid progenitor (MEP). In the mouse, a primitive MEP has been demonstrated in the yolk sac. In human, it is not known whether the primitive MK and erythroid lineages are generated from a common progenitor or independently. Using hematopoietic differentiation of human embryonic stem cells on the OP9 cell line, we identified a primitive MEP in a subset of cells coexpressing glycophorin A (GPA) and CD41 from day 9 to day 12 of coculturing. This MEP differentiates into primitive erythroid (GPA+CD41–) and MK (GPA–CD41+) lineages. In contrast to erythropoietin (EPO)–dependent definitive hematopoiesis, KIT was not detected during erythroid differentiation. A molecular signature for the commitment and differentiation toward both the erythroid and MK lineages was detected by assessing expression of transcription factors, thrombopoietin receptor (MPL) and erythropoietin receptor (EPOR). We showed an inverse correlation between FLI1 and both KLF1 and EPOR during primitive erythroid and MK differentiation, similar to definitive hematopoiesis. This novel MEP differentiation system may allow an in-depth exploration of the molecular bases of erythroid and MK commitment and differentiation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Stem cells unscramble yolk sac hematopoiesis Paul Gadue and Mitchell J. Weiss Blood 2009 114: 1455-1456. [Abstract] [Full Text] [PDF] This article has been cited by other articles: P. Gadue and M. J. Weiss Stem cells unscramble yolk sac hematopoiesis Blood, August 20, 2009; 114(8): 1455 - 1456. [Abstract] [Full Text] [PDF]

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