Blood, 1957, Vol. 12, No. 1, pp. 29-41.
© 1957 American Society of Hematology, Inc.
Immunologic Features of Erythrocyte Sensitization
II. The Nature of Blood Group Antibodies
JOHN H. VAUGHAN 1 and
MARION V. WALLER 1
1 Department of Medicine, Medical College of Virginia, Richmond.
It has been shown that red cells sensitized with anti-Rh antibody are agglutinated by anti-
-globulin sera, regardless of the "order" of Rh antibody activity.
Red cells sensitized with anti-Lewis antibody, on the other hand, are agglutinated by anti-sera to non-
-globulin substances. Of six other blood group antibodies studied, five provided
-globulin red cell coating materials and one, an
anti-Kidd, a non-
-globulin material. The participation of complement, or
complement-like, substances was shown in some instances.
Various pitfalls in the technics employed in absorbing Coombs sera so as to
render them immunochemically specific are discussed.
Submitted on April 16, 1956
Accepted on May 26, 1956