Blood, 1958, Vol. 13, No. 10, pp. 1001-1005.
© 1958 American Society of Hematology, Inc.
An Improved Method for Isolating Viable Human
Leukocytes from Peripheral Blood
JOSEPH H. LAPIN 1,
ANDREW HORONICK 1, and
ROBERT H. LAPIN 1
1 Institute of Geriatrics, Division of the Home and Hospital of the Daughters
of Jacob, Bronx, N.Y.
In an effort to isolate human leukocytes as free as possible of erythrocytes,
a two-stage method has been studied. The initial step, sedimentation of the
erythrocytes with Dextran results in a diminution of RBC:WBC ratio from
the 1000:1 of peripheral blood, to 2.34:1. After sedimentation in isotonic
saline, the RBC:WBC ratio is decreased to 0.16:1.
This method should prove valuable in preparing leukocytes for immunologic
studies.
Submitted on March 22, 1957
Accepted on March 20, 1958
