Blood, 1960, Vol. 15, No. 5, pp. 698-705.
© 1960 American Society of Hematology, Inc.
Phosphate Partition in the Erythrocytes of Normal Newborn
Infants and Infants with Erythroblastosis Fetalis.
II. Quantitative Paper Chromatography
T. J. GREENWALT 1 and
V. E. AYERS 1
1 Milwaukee Blood Center, Milwaukee, Wis.
Two-dimensional paper chromatography was used to partition the acid-soluble phosphates in the erythrocytes of normal newborn infants, infants
with erythroblastosis fetalis and adults.
The inorganic phosphate concentration was higher in the fresh red cells of
normal and erythroblastotic infants and rose more during incubation for four
hours than in the cells of adults.
The amount of erythrocyte adenosine-5’-triphosphate in the adults exceeded
the quantities found in the cells of the two groups of babies.
The 2,3-diphosphoglycerate level was greater in the erythrocytes of adults
and infants with erythroblastosis than it was in the normal infants and incubation for four hours resulted in a sharper decrease of this compound in cells
of the normal and erythroblastotic infants than in the adults.
