Blood, 1960, Vol. 16, No. 6, pp. 1708-1721.
© 1960 American Society of Hematology, Inc.
A Comparative Study of Some Staining Properties of
Crystals in a Lympho-plasmocytoid Cell, of Russell
Bodies in Plasmocytes, and of Amyloids-With
Special Emphasis on Their Isoelectric Points
ARTHUR F. GOLDBERG 1 and
HELEN WENDLER DEANE 1
1 Departments of Pathology and Anatomy, Albert Einstein College of Medicine,
New York 61, N. Y.
A staining procedure for determining the approximate isoelectric points of
substances has been applied to tissue sections containing (a) intracytoplasmic
crystals in a lympho-plasmocytoid cell, (b) Russell bodies of plasmocytes, and
(c) secondary and primary amyloids. The same method was also applied to
sections of human gamma globulin.
The results indicate the similarity of Russell bodies, the crystals, and gamma
globulin, all of which showed an isoelectric range between pH 6 and 7.5.
Other staining methods employed tended to confirm this identity.
The discrepancies reported in the literature on the staining properties of
Russell bodies are discussed.
All of the amyloids tested had an apparent isoelectric point near pH 5.
Various suggestions in the literature about the nature and origin of amyloid
are summarized.
Submitted on June 23, 1960
Accepted on August 13, 1960
