Blood, 1961, Vol. 17, No. 1, pp. 83-96.
© 1961 American Society of Hematology, Inc.
pH Dependent Hemolytic Systems. I. Their Relationship
to Paroxysmal Nocturnal Hemoglobinuria
STANLEY YACHNIN 1,
MITSUKO T. LAFORET 1, and
FRANK H. GARDNER 1
1 Richard C. Curtis Hematology Laboratory, Peter Bent Brigham Hospital, and
the Department of Medicine, Harvard Medical School.
1. Treatment of normal human red cells with various proteolytic enzymes,
cholera vibrio filtrate, influenza virus, and periodate ion results in red cells
susceptible to acid hemolysis in compatible normal human serum.
2. The kinetics of hemolysis of these artificially altered red cells resembles
in many respects those observed in paroxysmal nocturnal hemoglobinuria
(PNH).
3. While the observed differences in behavior between these artificially
altered cells and PNH cells do not allow direct comparison, it is felt that
these models may offer some clues in the understanding of PNH cell hemolysis, and some insight into the nature of the red cell lesion in paroxysmal nocturnal hemoglobinuria.
Submitted on July 14, 1960
