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Blood, 1961, Vol. 18, No. 3, pp. 344-348.
© 1961 American Society of Hematology, Inc.


Studies of the Irradiation Protection Effect of Fetal Liver in Mice. II. Storage by Freezing

JOHN H. GITHENS 1, PAUL N. TSCHETTER 1, M. GIOVANNELLA MOSCOVICI 1, and WILLIAM E. HATHAWAY 1

1 Department of Pediatrics, University of Colorado Medical Center, Denver, Colo.

Various methods of freezing have been evaluated for the preservation of the radiation protection effect in fetal mouse liver. The best results were obtained with tissue frozen slowly in glycerol to -80 C., stored at -50 C. or lower, and thawed rapidly. Other variables such as the size of the tissue particles, the amount of serum in the freezing mixture, and the method of dilution did not influence the results.

Submitted on February 13, 1961
Accepted on June 26, 1961


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