Blood, 1964, Vol. 23, No. 4, pp. 494-501.
© 1964 American Society of Hematology, Inc.
Low Temperature Preservation of Mouse Lymphocytes with Dimethyl Sulfoxide
M. J. ASHWOOD-SMITH 1
1 U. S. Naval Radiological Defense Laboratory, San Francisco, Calif.
Mouse lymphocytes have been successfully preserved for 3 months at -196
C. in the presence of 15 per cent dimethyl sulfoxide. Other concentrations of
dimethyl sulfoxide or glycerol were less effective. The viability of the frozen
lymphocytes was assessed by phase contrast microscopy, protein synthesizing
activity and by the ability of these lymphocytes to produce splenomegaly when
injected with F1 hybrids.
Submitted on September 20, 1963
Accepted on October 10, 1963
