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Blood, 1965, Vol. 26, No. 3, pp. 296-308.
© 1965 American Society of Hematology, Inc.
The Effect of Differing Demands for Blood Cell
Production on DNA Synthesis by Hemopoietic
Colony-Forming Cells of Mice
A. J. BECKER 1,
E. A. MCCULLOCH 1,
L. SIMINOVITCH 1, and
J. E. TILL 1
1 Department of Medical Biophysics, University of Toronto, and The Ontario
Cancer Institute, Toronto, Ontario, Canada.
A technic capable of estimating the fraction of hemopoietic colony-forming progenitor cells in DNA synthesis in vivo has been described. The technic
is based on the ability of tritiated thymidine to inhibit the growth of those
colony-forming cells which, by virtue of their presence in the S-phase during
a 20-minute incubation in vitro, in the presence of 500 µc./ml. of H3TdR,
have incorporated large amounts of the nucleoside. The method has been
applied to transplanted colony-forming cells proliferating in spleens of heavily
irradiated recipients as well as to cells from normal fetal liver, normal marrow, and normal spleen. In situations where the hemopoietic system is expanding (fetal liver and regenerating transplants), a large fraction, 40-65
per cent, of the stem cells are in DNA synthesis. In the steady-state situations
(adult marrow and spleen), the fraction of cells in DNA synthesis is almost
imperceptible using this technic. It is concluded that control mechanisms
which govern the rate of hemopoiesis operate, at least in part, by altering
the generative cycle of blood-forming progenitor cells.
Submitted on October 13, 1964
Accepted on January 25, 1965
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