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Blood, 1966, Vol. 27, No. 4, pp. 460-469.
© 1966 American Society of Hematology, Inc.

Tissue Culture of Bone Marrow

II. Effect of Steroid Hormones on Hematopoiesis in Vitro

EDWARD H. REISNER JR. 1

1 St. Luke’s Hospital Center, New York, New York.

In cultures of normal human bone marrow in a medium of 20 per cent horse serum and balanced salt solution, estrone stimulated granulopoiesis; testosterone inhibited fibroblast formation markedly and stimulated erythropoiesis; prednisolone inhibited fibroblast formation completely and allowed continued differentiation of all blood elements in smaller numbers.

Submitted on April 6, 1965
Accepted on June 19, 1965


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