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Blood, 1968, Vol. 32, No. 3, pp. 469-472.
© 1968 American Society of Hematology, Inc.

Satisfactory Red Cell Viability with Slight Excess of Acid Citrate Dextrose

KLAUS MAYER 1 and JOSEPH D'AMARO 1

1 Blood Bank, Department of Medicine, Memorial Hospital for Cancer and Allied Diseases, New York, N. Y.

Platelet suspensions obtained from blood donors are improved by increased acidification. The simplest way to accomplish this is to collect less blood into the standard quantity of ACD. Since we have previously reported an impairment in red cell viability when blood is collected in a great excess of ACD, it became pertinent to test the survival of stored red cells collected in a "slight" excess of ACD.

The volume of the blood collected was lowered to 375 ml. in 75 ml. ACD (N. I. H. formula A). At this ratio the pH. was 6.5 which is sufficiently low to minimize platelet clumping. The red cells were separated, stored for 21 days at 4 C., and viability was tested by the 51Chromate method. The results showed adequate red cell survival for blood collected and stored in this manner.

Submitted on January 22, 1968
Accepted on March 27, 1968


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K. MAYER and A. B. LEY
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Ann Intern Med, May 1, 1970; 72(5): 711 - 714.
[Abstract] [PDF]


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