|
|
 Previous Article | Table of Contents | Next Article 
Blood, 1972, Vol. 39, No. 1, pp. 39-56.
© 1972 American Society of Hematology, Inc.
Turnover Rate of Normal Blood Lymphocytes and
Exchangeable Pool Size in Man, Calculated From Analysis
of Chromosomal Aberrations Sustained During
Extracorporeal Irradiation of the Blood
E. O. Field 1,
H. B. A. Sharpe 1,
K. B. Dawson 1,
V. Andersen 1,
S. A. Killmann 1, and
E. Weeke 1
1 Radiotherapy Research Unit, Institute of Cancer Research, Royal Marsden
Hospital, Sutton, Surrey, England, and the Divisions of Hematology and Nephrology,
Rigshospitalet, Blegdamsvej, Copenhagen, Denmark.
Lymphocyte kinetics have been
studied in four patients treated by
extracorporeal irradiation of the blood
(ECIB) for immunosuppressive purposes prior to renal transplantation.
The technique involves the analysis of
chromosomal aberrations sustained by
lymphocytes during irradiation and
demonstrable in cultures stimulated
with phytohaemagglutinin (PHA). Lymphocytes containing aberrations may
be regarded as labeled, and this form
of labeling has the advantage that it
does not require the manipulation of
lymphocytes outside the body. Preliminary experiments established that
about half of lymphocytes irradiated
with doses of 300-400 rad fail to undergo mitosis in culture and that the
aberration yield per lymphocyte is the
same at the end of a course of ECIB
as at the beginning. Irradiated lymphocytes were found to have a mean residence time in the blood of less than 2
min, and for the first few hours of
irradiation they were replaced by
lymphocytes from tissue pools at a
rate such that there was no appreciable fall in the peripheral count. These
findings imply that a large proportion
of the vascular endothelium acts as a
filter for damaged lymphocytes and
that initially damaged cells were replaced at the rate of one blood pool
per hour; this may represent the normal turnover time of blood lymphocytes. The size of the total exchangeable lymphocyte pool was calculated
by two methods depending on a series
of basic assumptions concerning the
kinetics of irradiated cells. According
to the most likely estimate, the total
pool contains some 30 times as many
cells as circulate in the blood. Forty-two per cent of lymphocytes survived
an irradiation dose of 300 rad, 10%-19% survived 380 rad.
Submitted on May 28, 1971
Revised on July 24, 1971
Accepted on July 27, 1971
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
C. S. Eby
Bleeding and Thrombosis Risks in Plasma Cell Dyscrasias
Hematology,
January 1, 2007;
2007(1):
158 - 164.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. E. Mager, S. X. Lin, R. A. Blum, C. D. Lates, and W. J. Jusko
Dose Equivalency Evaluation of Major Corticosteroids: Pharmacokinetics and Cell Trafficking and Cortisol Dynamics
J. Clin. Pharmacol.,
November 1, 2003;
43(11):
1216 - 1227.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
