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Next Article 
Blood, 1972, Vol. 40, No. 3, pp. 303-310.
© 1972 American Society of Hematology, Inc.
Increased Proportion of Complement-Receptor
Lymphocytes in the Peripheral Blood of Patients
With Chronic Lymphocytic Leukemia
Seth Pincus 1,
Celso Bianco 1, and
Victor Nussenzweig 1
1 Department of Pathology, New York University School of Medicine, New York,
N. Y.
In the present study we present evidence that the proportion of complement-receptor lymphocytes (CRL) is
greatly increased in the circulation
in most cases of chronic lymphocytic
leukemia (CLL). Lymphocytes (> 99%
pure, 70% recovery) were obtained
from the peripheral blood of normal
individuals by separation of the mononuclear cells from the leukocyte-enriched plasma by differential flotation
in Hypaque-Ficoll and incubation of
the mononuclear cells with iron-containing particles followed by removal
of the phagocytes with a magnet.
Complement - receptor lymphocytes
were detected by incubating lymphocytes with sheep erythrocytes coated
with antibody and mouse complement
(EAC) and counting the EAC—CRL
rosettes under the microscope. 7.1 ±
3.8% of normal peripheral blood
lymphocytes, 31.0 ± 6.9% of lymph
node, and 59.6 ± 13.2% of tonsil
lymphocytes bind EAC. The binding
was C3-dependent since it could be
inhibited specifically by papain fragments of rabbit antibodies to mouse
C3. Among lymphocytes from the peripheral blood of patients with CLL,
50.7 ± 25.0% bear the complement
receptor. These results suggest that
CLL preferentially affects B cells.
Submitted on January 21, 1972
Revised on March 28, 1972
Accepted on April 18, 1972
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