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Blood, 1973, Vol. 42, No. 3, pp. 413-421.
© 1973 American Society of Hematology, Inc.
Cholinesterase as a Possible Marker for Early Cells
of the Megakaryocytic Series
Carl W. Jackson 1
1 Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tenn.
Among marrow cells of rats only megkaryocytes stain appreciably for acetylcholinesterase. An investigation was
therefore undertaken to determine whether
this staining characteristic might be used
to identify precursors of recognizable
megakaryocytes. Marrow cells smaller
than recognizable megakaryocytes were
positive for the enzyme. The number of
these cells increased after induction of
severe thrombocytopenia by injection of
antiplatelet antiserum. Subsequently, the
number of recognizable megakaryocytes
increased, suggesting their derivation from
the small acetylcholinesterase-positive
cells. These results suggest that acetylcholinesterase activity can be used as a
marker for megakaryocytic cells that cannot be detected with conventional staining
methods.
Submitted on January 25, 1972
Revised on December 28, 1972
Accepted on February 7, 1973
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