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Blood, 1974, Vol. 43, No. 4, pp. 591-595.
© 1974 American Society of Hematology, Inc.


Separation of Megakaryocytes From Mouse Bone Marrow by Velocity Sedimentation

A. Nakeff 1 and B. Maat 1

1 Department of Radiobiology, Medical Faculty, Rotterdam, and the Radiobiological Institute TNO, Rijswijk, The Netherlands.

A technique for separating mouse bone marrow megakaryocytes by unit gravity velocity sedimentation is described using a modified sedimentation chamber. The velocity sedimentation profile for megakaryocytes comprises three peaks corresponding to sedimentation velocities of 30, 60, and 100 mm/hr. Morphologic examination of megakaryocytes in the 30 mm/hr peak reveals them to be primarily immature with the latter two peaks corresponding to larger, more mature megakaryocytes.

Submitted on January 3, 1973
Revised on August 17, 1973
Accepted on August 27, 1973


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