Blood, 1974, Vol. 43, No. 4, pp. 591-595.
© 1974 American Society of Hematology, Inc.
Separation of Megakaryocytes From Mouse
Bone Marrow by Velocity Sedimentation
A. Nakeff 1 and
B. Maat 1
1 Department of Radiobiology, Medical Faculty, Rotterdam, and the Radiobiological Institute TNO, Rijswijk, The Netherlands.
A technique for separating mouse bone
marrow megakaryocytes by unit gravity
velocity sedimentation is described using
a modified sedimentation chamber. The
velocity sedimentation profile for megakaryocytes comprises three peaks corresponding to sedimentation velocities of
30, 60, and 100 mm/hr. Morphologic
examination of megakaryocytes in the 30
mm/hr peak reveals them to be primarily
immature with the latter two peaks corresponding to larger, more mature megakaryocytes.
Submitted on January 3, 1973
Revised on August 17, 1973
Accepted on August 27, 1973