In vitro production of erythropoietin by mouse fetal liver
JR Zucali, V Stevens and EA Mirand
Mouse fetal liver tissue has been cultured and shown to produce and release
into the culture medium an erythropoietically active substance for up to 30
days of culture. Since this substance can be completely neutralized by an
antiserum to erythropoietin and shows a dose-- response relationship in the
plethoric mouse assay, it is suggested that the culture medium contains
erythropoietin, a hormone important in the regulation of erythropoiesis.
Using this procedure, we have obtained the equivalent of about 20.7 unites
of erythropoietin from five T-flasks (75 sq cm) over the 30-day culture
period.
Volume 46,
Issue 1,
pp. 85-90,
07/01/1975
Copyright © 1975 by The American Society of Hematology