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S Murphy and FH Gardner
Containers constructed of polyvinylchloride (PVC) are used for the storage
of platelet concentrates (PC) for transfusion, At 22 degrees C, pH often
falls to such low levels (pH is less that 6.0) that viability is lost. Far
lesser degrees of pH fall are observed in bags constructed of polyethylene
(PE). In this study, pH, PO2, PCO2, platelet count, lactate concentration,
microscopic morphology, and viability after 51- chromium labeling were
evaluated during storage at 22 degrees C under a variety of circumstances.
The results indicate that (1) pH falls because of the generation of lactic
acid by platelet glycolysis and, under some circumstances, the retention of
CO2. (2) Rate of pH fall is, therefore, roughly proportional to the
platelet count. (3) PE is more permeable to gases, thereby allowing CO2
escape from and easier O2 entry into the stored PC; the higher O2 tensions
suppress glycolysis by the Pasteur effect. (4) Adequate agitation and
container size are critical if the beneficial effect of PE is to be
obtained. (5) In general, platelets stored in PE containers have excellent
viability in vivo although CO2 escape can result in elevations in pH which
are deleterious. (6) Storage in a 10% CO2 atmosphere prevents these
deletrrious pH elevations without otherwise impairing platelet viability;
(7) Results similar to those achieved with PE can be achieved with PVC if
this material is made thinner to allow easier penetration of gases
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| Copyright © 1975 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
