A measurement technique for cell adhesiveness
IT Young and SE Sher
Determination of the percentage of cells in clumps on a stained smear of
human peripheral blood porvided a useful, accurate technique for measuring
cell adhesiveness. Smears of human peripheral blood drawn with EDTA were
prepared on a blood slide centrifuge, stained, and examined under a light
microscope. Statistical analysis showed that the method resulted in a
Poisson distribution of particles on the slide, where a particle was
considered to be a simple cell, or two or more cells which appeared to be
touching, Analysis of the distributions of erythrocytes and leukocytes
showed that clumps were formed before the cells were deposited on the
slide. When adhesiveness of erythrocytes or leukocytes was increased by
incubation with antiserum to the corresponding cell type, the percentage of
that cell type in clumps increased proportionately, Preliminary results
using the method showed that normal human donors had similar to 1% of their
erythrocytes and 1- 5% of their leukocytes in clumps. In chronic myelocytic
leukemia, as many as 60% of the leukocytes were in clumps.
Volume 46,
Issue 2,
pp. 287-294,
08/01/1975
Copyright © 1975 by The American Society of Hematology
