Heterogeneity of human circulating anticoagulants against antihemophilic
factor (factor VIII)
MC Poon, AC Wine, OD Ratnoff and GM Bernier
The heterogeneity of human circulating anticoagulants against
antihemophilic factor (AHF, factor VIII) observed in seven patients, both
with and without classic hemophilia, was investigated by neutralization of
their activity with antiserums directed to whole IgG and to lambda and
kappa light chains. All seven anticoagulants were immunoglobulins. Six
appeared to contain both kinds of light chains, although the dual light
chain composition of two of these could be demonstrated only at high
concentration of antiserum. In one circulating anticoagulant, light chain
specificity could not be demonstrated with small amounts of antiserum, and
with larger amounts, only lambda light chain specificity was revealed.
Whether or not this circulating anticoagulant really contained a single
light chain type could not be ascertained with our technique. The evidence
presented suggested that circulating anticoagulant antibodies against AHF
are polyclonal in nature.
Volume 46,
Issue 3,
pp. 409-416,
09/01/1975
Copyright © 1975 by The American Society of Hematology
