Cryoprotective agents as inducers of erythroleukemic cell differentiation
in vitro
HD Preisler, G Christoff and E Taylor
The ability of families of compounds with known and potential
cryoprotective properties to induce the differentiation of Friend leukemia
cells in vitro was studied. For each agent, both the proportion of
differentiated cells in the culture and the total amount of heme/10(7)
cells were determined. Within each family of compounds there was a direct
correlation between a compound's cryoprotective ability, its ability to
donate electron pairs for hydrogen bonding (basicity), and its ability to
induce differentiation. While individual agents differed with respect to
the proportion of cells which were induced to differentiate, the biology of
the process of differentiation appeared to be similar, regardless of the
agent used. A cell line which was unresponsive to DMSO was responsive to
other inducers, suggesting that this DMSO-resistant cell line differed from
its parent DMSO- responsive cell line either in its metabolism of the
inducers or in the ability of the inducers to enter the cell.
Alternatively, there may be more than one mechanism involved in the
chemical induction of differentiation.
Volume 47,
Issue 3,
pp. 363-368,
03/01/1976
Copyright © 1976 by The American Society of Hematology
