Improved techniques for liquid culture of human and mouse bone marrow
MJ Cline and DW Golde
Previous studies using the in vitro diffusion chamber (Marbrook) have shown
that bone marrow grown in this system will undergo limited stem cell
replication and differentiation to mature granulocytes and mononuclear
phagocytes. A series of studies with modified culture systems was initiated
to improve cell production and committed stem cell (CFU-C) proliferation in
vitro. Introduction of a continuous-flow system and a migration technique
providing means of egress for mature neutrophils resulted in substantially
improved performance. CFU-C were found to be capable of migration through a
3-mu pore membrane. These studies indicated that membrane surface area,
culture medium circulation, and mature cell egress were among the
conditions that could be optimized for maximum hematopoietic cell
proliferation in suspension culture. The present observations also
suggested that large- scale in vitro growth of mammalian bone marrow may be
feasible.
Volume 47,
Issue 3,
pp. 369-379,
03/01/1976
Copyright © 1976 by The American Society of Hematology
