Production of colony-stimulating factor by leukemic leukocytes
JM Goldman, KH Th'ng, D Catovsky and DA Galton
The production of colony-stimulating factor (CSF) by the peripheral blood
cells of untreated patients with acute myeloid leukemia (AML) was measured
in the agar culture system using normal human bone marrow as the source of
colony-forming units (CFUc). CSF production was found to be variable and
was related to the morphologic subtype of AML--cells from patients with
monocytic leukemia produced normal or large quantities of CSF, while (with
one exception) those from patients with myeloblastic leukemia produced
little or no CSF. There was a general relationship between CSF production
and serum lysozyme levels. Attempts to demonstrate a consistent inhibitory
effect exerted by leukemic peripheral blood cells on normal leukopoiesis in
vitro were negative. Results instead suggested that the addition to the
feeder layer of cells from patients with monocytic leukemia could raise CSF
levels above those obtained with normal peripheral blood leukocytes alone,
possibly by recruiting additional CFUc from normal marrow.
Volume 47,
Issue 3,
pp. 381-388,
03/01/1976
Copyright © 1976 by The American Society of Hematology
