Sterospecific tissue uptake and nuclear accumulation of testosterone in the
development of the mouse erythropoietic spleen
AJ Hadjian, JL Spivak, A Kowarski, HW Dickerman and CJ Migeon
A double isotope ratio technique was used to estimate the specific binding
of testosterone (T), as opposed to its biologically nonactive stereoisomer,
epitestosterone (Epi T). The mouse erythropoietic spleen formed in response
to a phenylhydrazine-induced hemolytic anemia was used as the target organ.
Spleen minces from preanemic mice, as well as those in the early and late
phases of erythropoietic spleen development, were incubated with 10(-9) M
of 14C-T and 3H-EpiT, and the selective uptake of T was calculated from the
14C/3H ratio measured in the media before and after incubation, as well as
in the subcellular fractions of the minces. Preferential uptake of T was
seen in the early phase of development, but not in spleens obtained from
preanemic animals or those in the late phase. There was no evidence of
metabolic conversion of T or EpiT. The selective uptake of T by early phase
spleens was reflected in a preferential nuclear accumulation of T. These
data represent the first demonstration of a specific binding of T in vitro
to a developing erythroid tissue.
Volume 47,
Issue 4,
pp. 571-580,
04/01/1976
Copyright © 1976 by The American Society of Hematology
