SEARCH:   Advanced

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Steinberg, H. N. Articles by Handler, E. E. Search for Related Content PubMed PubMed Citation Articles by Steinberg, H. N. Articles by Handler, E. E. Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Assessment of erythrocytic and granulocytic colony formation in an in vivo plasma clot diffusion chamber culture system HN Steinberg, ES Handler and EE Handler Normal rat bone marrow cells seeded into a plasma clot diffusion chamber culture developed into erythrocytic and granulocytic colonies in vivo. Chambers implanted into the peritoneal cavity of normal hosts showed erythrocytic colony numbers reaching an initial peak on day 2, declining on days 3--5, and increasing in a secondary growth phase on day 7. Day 2 colonies were evenly dispersed; day 7 colonies were grouped into discrete areas of bursts. Granulocytic colony numbers reached a peak on day 4 and gradually declined through day 7. Cells in various stages of differentiation could be detected in both colony types. Colony numbers were proportional to the number of marrow cells seeded into the chamber. Host animals treated with phenylhydrazine induced a marked increase in erythrocytic colony numbers and size and a decrease in granulocytic colony formation. Host animals treated with endotoxin suppressed erythrocytic colonies while increasing granulocytic colony size. This method may prove advantageous for the study of hematopoietic colony formation in a physiologic environment. Volume 47, Issue 6, pp. 1041-1051, 06/01/1976 Copyright © 1976 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: G Van Zant and E Goldwasser Simultaneous effects of erythropoietin and colony-stimulating factor on bone marrow cells Science, November 18, 1977; 198(4318): 733 - 735. [Abstract] [PDF]

	Copyright © 1976 by American Society of Hematology         Online ISSN: 1528-0020