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Next Article 
Enrichment of erythrocytes of fetal origin from adult-fetal blood mixtures
via selective hemolysis of adult blood cells: an aid to antenatal diagnosis
of hemoglobinopathies
SH Boyer, AN Noyes and ML Boyer
Red cell lysis in isotonic solutions containing NH4Cl, NH4HCO3, and a
carbonic anhydrase enzyme inhibitor (acetazolamide) is a function of
erythrocyte enzyme activity and permeability of cells to the inhibitor.
Erythrocyte carbonic anhydrase activity is at least fivefold greater and
acetazolamide permeability about tenfold less for adults than for newborns.
In this setting, greater than 99.9% of red cells from adults can be
hemolyzed at a time when greater than 25% of those from newborns remain
intact. This easily applied method may be useful when antenatal diagnosis
of hemoglobinopathies is otherwise precluded by contaimination with
maternal erythrocytes. The feasibility of differential hemolysis via
NH4Cl--HCO3-mediated, acetazolamide- modulated reactions is shown by the
successful isolation of the few fetal-origin erythrocytes present in
grossly nonbloody amniotic fluids and, in one instance, by approximately
3300-fold enrichment of apparently authentic fetal-origin red cells from
the arm blood of a woman in her 18th wk of pregnancy.
Volume 47,
Issue 6,
pp. 883-897,
06/01/1976
Copyright © 1976 by The American Society of Hematology
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