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R Largo, V Heller and PW Straub
The presence of minimal amounts of fibrinogen-fibrin intermediates in human
plasma was visualized by an agglutination reaction of
glutaraldehyde-treated human erythrocytes coated with purified fibrin
monomers. A degree of monomer coating was established which produced
erythrocytes not agglutinated by normal plasma but by plasma containing
minimal amounts of soluble complexes of fibrinogen with fibrin monomers.
Under standardized conditions of coating, erythrocyte concentration,
temperature, pH, and incubation time, the agglutination time varied with
the ratio of soluble fibrin to fibrinogen in plasma. The test was sensitive
down to a soluble fibrin concentration of 0.675% of the plasma fibrinogen
concentration. Early fibrinogen and fibrin degradation products (FDP) in
the plasma led to a prolongation of the agglutination time at a
concentration of more than 16 mg/100 ml. Late FDP in a concentration of 100
mg/100 ml did not convert a positive test to negative. The test was not
affected by heparin and protamine at concentrations of up to 12.5 and 50
NIH units/ml, respectively.
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| Copyright © 1976 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
