Comparison of normal and chronic lymphocytic leukemia lymphocyte surface Ig
determinants using peroxidase-labeled antibodies. II. quantification of
light chain determinants in atypical lymphocytic leukemia
G Dighiero, JY Follezou, JP Roisin, T Ternynck and JL Binet
Five cases of atypical lymphocytic leukemia were investigated with regard
to their membrane-associated light chains. Detection and quantitation of
antigenic determinants were performed by means of peroxidase-labeled
antibodies according to Avrameas et al. The cases studied had clinical and
cytologic features in common: an active clinical course, marked
splenomegaly, severe anemia and thrombocytopenia, little or no lymph node
enlargement, and very high white blood counts with small mature lymphocytes
and poorly differentiated lymphoid cells. Blood lymphocytes of all patients
carried a single type of light chain, and 90%-100% of the cells were
stained. The average number of antigenic sites per cell was 72,500 (range
40,000-97,500). These results differed from those previously found in
typical CLL (mean value 9000) and approached the values of normal
peripheral blood lymphocytes (90,000). The criteria investigated in this
study could be of value for the diagnosis and prognosis of some atypical
forms of lymphocytic leukemia.
Volume 48,
Issue 4,
pp. 559-566,
10/01/1976
Copyright © 1976 by The American Society of Hematology
