Granulocyte function in chronic granulocytic leukemia. II. Bactericidal
capacity, phagocytic rate, oxygen consumption, and granule protein
composition in isolated granulocytes
T Olofsson, H Odeberg and I Olsson
The initial rate of phagocytosis, oxygen consumption rate during
phagocytosis, bactericidal capacity against Escherichia coli, and the
granule protein composition of isolated mature-appearing granulocytes were
studied in 23 patients with chronic granulocytic leukemia (CGL) with the
simultaneous use of normal controls. The initial rate of phagocytosis was
decreased (p less than 0.05) in the CGL patient group, as were oxygen
consumption rate (p less than 0.001) and bactericidal capacity (p less than
0.001). Kinetic analysis of the ingestion rate showed CGL granulocytes to
have the same capacity to bind the particles as normal granulocytes. Both
specific and primary granule protein deficiencies were shown for CGL
granulocytes, and these deficiencies were more pronounced at or near blast
cell transformation. Analysis of all different granulocyte function
parameters showed an inverse correlation to white blood cell counts (p less
than 0.01) and to the percentage of immature granulocytes in peripheral
blood (p less than 0.001). The leukocytosis doubling time was progressively
shortened during the chronic course of the disease. A correlation was found
between granulocyte function parameters and leukocytosis doubling time (p
less than 0.001), indicating that granulocyte function was progressively
deteriorating during chronic phase CGL, and may be an expression of
increasing disturbance of the differentiation process.
Volume 48,
Issue 4,
pp. 581-593,
10/01/1976
Copyright © 1976 by The American Society of Hematology
