Phosphorylation in erythrocyte membranes from abnormally shaped cells
AC Greenquist and SB Shohet
Erythrocyte protein phosphorylation was examined in membrane preparations
of 25 patients with hereditary spherocytosis (HS). Reduced phosphorylation
in substrate polypeptides was observed in 22 HS erythrocyte membranes for
both splenectomized and nonsplenectomized patients. A reduction in labeling
was also observed in a polypeptide which was labeled only in the presence
of cAMP. No reduction was observed in membranes from immunologically
acquired spherocytosis cells or from cells from patients with hereditary
elliptocytosis. Heating membranes to 45 degrees C caused negligible
inhibition of membrane protein phosphorylation, while heating to 50 degrees
C extensively inhibited phosphorylation. Dephosphorylation of membrane
protein that occurred in isolated membranes was not dependent upon cAMP.
Volume 48,
Issue 6,
pp. 877-886,
12/01/1976
Copyright © 1976 by The American Society of Hematology
