Interacting cell populations in cultures of leukocytes from normal or
leukemic peripheral blood
Kinetic studies in cultures containing 2 X 10(5) peripheral leukocytes from
patients with acute myelobastic leukemia revealed extensive,
radiation-sensitive increases in thymidine incorporation without parallel
increases in cell number. Modest and variable stimulation of 3HTdR
incorporation was seen with the addition of either leukocyte- conditioned
medium prepared with phytohemagglutin (PHA) or PHA alone. However, using
the method of limiting dilution, stimulation was always observed and ranged
from 3- to 20-fold in individual patients. By mixing small numbers of
intact cells with larger numbers of irradiated autologous cells,
quantitative evidence was obtained for a cellular interaction between
irradiated, PHA-stimulated populations capable of 3HTdR incorporation.
Similar evidence for cell-cell interaction was obtained for normal
leukocytes.
Volume 49,
Issue 2,
pp. 269-280,
02/01/1977
Copyright © 1977 by The American Society of Hematology
