Effect of cytochalasin B on human monocyte binding and sphering of IgG-
coated human erythrocytes
B Herskovitz, D Guerry , RA Cooper and AD Schreiber
The ability of human mononuclear phagocytic cells to bind IgG-coated human
erythrocytes (EA) and to cause bound EA to become osmotically fragile
(sphered) was investigated in the presence of cytochalasin B, a known
inhibitor of phagocytosis. Cytochalasin B inhibited the binding of EA to
mononuclear cells in a dose-dependent fashion; 80% inhibition of binding
was observed at a concentration of 5 mug/ml. This profound effect on EA
binding together with presently available data suggested a role for IgG
receptor mobility in the macrophage binding of IgG-coated erythrocytes.
Cytochalasin B, however, had a minimal effect on the capacity of
mononuclear cells to sphere adherent EA, suggesting that the processes
involved in macrophage-induced spherocytosis may differ from those operable
in phagocytosis.
Volume 49,
Issue 2,
pp. 289-294,
02/01/1977
Copyright © 1977 by The American Society of Hematology
