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Next Article 
Human marrow cells capable of erythropoietic differentiation in vitro:
definition of three erythroid colony responses
CJ Gregory and AC Eaves
A systematic study has been undertaken to analyze the spectrum of
erythropoietic colonies obtained in cultures of human marrow cells plated
in methyl cellulose. Colonies were identified as erythropoietic on the
basis of the appearance in them of hemoglobin-containing erythroblasts. As
found previously in mouse marrow cultures, three sequentially appearing
types of colonies which differed in their ultimate cluster content could be
readily distinguished. Small erythroid colonies containing 1-2 clusters
reached a peak after 7-8 days; small bursts containing 3-8 clusters reached
a peak after 10-12 days; and large bursts containing greater than 16
clusters reached a peak after 17-20 days. The previously reported enhancing
effect of human leukocyte conditioned medium on burst formation seen in
cultures of human nonadherent cells was found to be due largely to an
effect on the formation of the largest, late appearing type of burst. By
analogy with the mouse, the progenitors of such bursts would represent a
primitive cell type which has a close relationship with pluripotent stem
cells, as well as a second and independent close relationship to the
progenitors of granulopoietic colonies.
Volume 49,
Issue 6,
pp. 855-864,
06/01/1977
Copyright © 1977 by The American Society of Hematology
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