|
|
 Previous Article | Table of Contents | Next Article 
Tissue-factor coagulant activity of cultured human endothelial and smooth
muscle cells and fibroblasts
JR Maynard, BE Dreyer, MB Stemerman and FA Pitlick
The tissue-factor (thromboplastic) activity of cultured human endothelial
cells and fibroblasts is low at time of transfer into fresh medium but
increases 3-10 fold. Endothelial cells reach peak activity (400 U/10(5)
cells) 5-8 hr after subculture. Activity in fibroblast cultures peaks
(3000-12,000 U/10(5) cells) 7-12 hr after subculture. After attaining
maximum activity, endothelial and fibroblast tissue- factor content
decreases in a time course similar to other cells studied in this
laboratory, approaching basal levels by 24-50 hr after subculture. If
medium over fibroblasts is changed every 12 hr, activity can be sustained
at the peak level for an additional day but cannot be maintained at a high
level indefinitely. The kinetics of expression of smooth muscle cell tissue
factor are markedly different from other cell types. There is always a
pronounced lag (30 hr or more) before the activity increases, and then, in
most cases, there is no subsequent decline in activity even though the
cells are not refed or restimulated. The activity of each of these cell
types is cryptic but becomes available after freeze-thaw disruption of
cells.
Volume 50,
Issue 3,
pp. 387-396,
09/01/1977
Copyright © 1977 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
S. McCoy and K. Baldwin
Pharmacotherapeutic options for the treatment of preeclampsia
Am. J. Health Syst. Pharm.,
February 15, 2009;
66(4):
337 - 344.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. K. Mandal, U. R. Pendurthi, and L. V. M. Rao
Cellular localization and trafficking of tissue factor
Blood,
June 15, 2006;
107(12):
4746 - 4753.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. H. Yamani, C. S. Masri, N. B. Ratliff, M. Bond, R. C. Starling, E. M. Tuzcu, P. M. McCarthy, and J. B. Young
The role of vitronectin receptor ({alpha}v{beta}3) and tissue factor in the pathogenesis of transplant coronary vasculopathy
J. Am. Coll. Cardiol.,
March 6, 2002;
39(5):
804 - 810.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W Li and G Keller
VEGF nuclear accumulation correlates with phenotypical changes in endothelial cells
J. Cell Sci.,
January 5, 2000;
113(9):
1525 - 1534.
[Abstract]
[PDF]
|
|
|
|
|
|
|
M. H. A. M. Veltrop, H. Beekhuizen, and J. Thompson
Bacterial Species- and Strain-Dependent Induction of Tissue Factor in Human Vascular Endothelial Cells
Infect. Immun.,
November 1, 1999;
67(11):
6130 - 6138.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Kaikita, H. Ogawa, H. Yasue, M. Takeya, K. Takahashi, T. Saito, K. Hayasaki, K. Horiuchi, A. Takizawa, Y. Kamikubo, et al.
Tissue Factor Expression on Macrophages in Coronary Plaques in Patients with Unstable Angina
Arterioscler Thromb Vasc Biol,
October 1, 1997;
17(10):
2232 - 2237.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
R. R. Bach and C. F. Moldow
Mechanism of Tissue Factor Activation on HL-60 Cells
Blood,
May 1, 1997;
89(9):
3270 - 3276.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Kirchhofer, T. B. Tschopp, and H. R. Baumgartner
Active Site–Blocked Factors VIIa and IXa Differentially Inhibit Fibrin Formation in a Human Ex Vivo Thrombosis Model
Arterioscler Thromb Vasc Biol,
August 1, 1995;
15(8):
1098 - 1106.
[Abstract]
[Full Text]
|
|
|
|
|
