Erythroid cell growth from normal and W/WV murine bone marrow on
macrophage-coated membranes
BJ Torok-Starb, NS Wolf and DR Boggs
Cellulose acetate membranes (CAM) placed in the peritoneal cavity of mice
develop a macrophage layer capable of supporting in vivo hematopoietic
colonies from intraperitoneally injected bone marrow cells. Modifications
allowing for routine morphologic identification of colonies showed that
both erythrocytic (E) and granulocytic (G) colonies occur with a consistent
E:G ratio of 0.19 +/- 0.037. Stimulating recipients by bleeding or
phenylhydrazine injection did not produce a significant change in the total
number of colonies and a reduction in granulocytic colonies so that the E:G
ratio significnatly increased. Hypertransfusion of donor animals had no
effect on the number of erythroid colonies that grew on CAM of average
recipients. The total colony-forming ability of bone marrow cells from
genetically anemic W/WV mice was found not to differ from that of normal
+/+ littermates; however, the E:G ratio of W/WV marrow in bled recipients
was significantly lower (p less than 0.01) then that of +/+ marrow. These
studies suggest that a CAM system supports an erythroid progenitor which is
not affected by hypotransfusion of the donor animal, yet is dependent upon
erythropoietin for colony formation, and that it is defective in the W/WV
mouse.
Volume 50,
Issue 5,
pp. 857-866,
11/01/1977
Copyright © 1977 by The American Society of Hematology
