Stimulation of the hexose monophosphate shunt independent of hydrogen
peroxide and superoxide production in rabbit alveolar macrophages during
phagocytosis
MF Tsan
Phagocytosis and oxidative metabolism of human polymorphonuclear leukocytes
(PMN) and rabbit alveolar macrophages (AM) were studied. Human PMN ingested
a mean of 12 polyvinyl toluene latex particles (2 micrometer in diameter)
per cell. There was stimulation of O2- and H2O2 production, light emission,
and activation of the hexose monophosphate shunt during phagocytosis by
human PMN. Rabbit AM ingested 51 latex particles (2 micrometer in diameter)
per cell. There was no stimulation of the production of O2- and H2O2 or
light emission associated with phagocytosis by rabbit AM, while the hexose
monophosphate shunt was activated. Similar metabolic changes were obtained
in both cell types when opsonized zymosan was used as phagocytic particles.
1-14C- glucoseoxidation was stimulated by H2O2 and methylene blue in both
resting human PMN and rabbit AM. It is concluded that activation of the
hexose monophosphate shunt in rabbit AM during phagocytosis is independent
of O2- and H2O2 production.
Volume 50,
Issue 5,
pp. 935-945,
11/01/1977
Copyright © 1977 by The American Society of Hematology
