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Defective iron uptake and globin synthesis by erythroid cells in the anemia
of the Belgrade laboratory rat
JA Edwards, LM Garrick and JE Hoke
Erythroid cell iron uptake and globin synthesis were studied in the anemia
of the Belgrade Laboratory rat (gene symbol, b), an autosomal recessive
trait characterized by hypochromia and hyperferrinemia. Reticulocyte
protein and globin synthesis, as measured in vitro by the incorporation of
3H-L-leucine, were significantly diminished in b/b animals, although no
major imbalance between alpha-and beta-chain production was observed in b/b
reticulocytes. The incorporation in vitro of 3H-L-methionine into marrow
cell globin demonstrated no difference between b/b animals and +/? control
animals in the proportion of alpha-to beta-chain production. The transfer
of iron from plasma to reticulocytes, as measured in vitro by the uptake of
59Fe, was significantly decreased in b/b animals; Sephadex G 200
chromatography of b/b red cell lysates did not reveal the accumulation of
59Fe in nonhemoglobin fractions found when heme synthesis was inhibited
with isoniazid. The magnitude of the reticulocyte iron-uptake defect was
greater than the reticulocyte globin-synthesis defect, suggesting that the
former is the primary lesion.
Volume 51,
Issue 2,
pp. 347-357,
02/01/1978
Copyright © 1978 by The American Society of Hematology
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