Discrimination between human T and B lymphocytes and monocytes by computer
analysis of digitized data from scanning microphotometry. I. Chromatin
distribution patterns
BG Durie, L Vaught, YP Chen, GB Olson, SE Salmon and H Bartels
Digitized images of Feulgen-stained normal human B and T lymphocytes and
monocytes were analyzed by computer and microphotometry. The algorithms
assessed the chromatin distribution patterns. A substantial number of
parameters descriptive of the chromatin distributions were found to show
statistically highly significant differences between B and T cells and
monocytes. Although the effects of a number of biologic variables, such as
cell cycle phase and immune stimulation, need to be carefully evaluated,
the preliminary results indicate potential for automatic classification and
subclassification of human B and T lymphocytes in normal and disease
states.
Volume 51,
Issue 4,
pp. 579-589,
04/01/1978
Copyright © 1978 by The American Society of Hematology
