Platelet factor VIII-related antigen: studies in vivo after transfusion in
patients with von Willebrand disease
Y Sultan, C Jeanneau, J Lamaziere, P Maisonneuve and JP Caen
Four unrelated patients with a severe form of von Willebrand disease showed
no detectable factor VIII-related antigen (VIIIR:AG) in either their plasma
or their platelets. They received cryoprecipitate infusions, three patients
in a single injection each and one every day for 9 days before and after
surgery. Platelet VIIIR:Ag was studied at different times during and after
transfusion using electroimmunoassay of platelet extracts and electron
microscopy of the platelets incubated with anti-VIIIR:Ag antibodies coupled
to peroxidase. No VIIIR-Ag was detected in or around the patients'
platelets, although this antigen was detected in the circulating blood.
These results that there was no VIIIR:Ag uptake from the plasma by the
platelets and that platelet VIIIR:Ag came from megakaryocytes.
Volume 51,
Issue 4,
pp. 751-761,
04/01/1978
Copyright © 1978 by The American Society of Hematology
