Polyamine synthesis in bone marrow granulocytes: effect of cell maturity
and early changes following an inflammatory stimulus
WH Evans, CK Grieshaber, WC Miller, SM Wilson and HA Hoffman
Enriched fractions of mature and immature neutrophil granulocytes, isolated
from guinea pig bone marrow, were assayed for ornithine decarboxylase
activity and polyamine content. The results show that immature granulocytes
contain at least ten times more ornithine decarboxylase activity and two
times more spermidine than mature granulocytes. The incorporation of
14C-ornithine into putrescine and spermidine of intact immature
granulocytes was three to four times and ten times, respectively, that of
mature granulocyte preparations. Six hours after an inflammatory stimulus,
transient increases of 14-fold and 3-fold in the activities of ornithine
decarboxylase and S-adenosyl- L-methionine decarboxylase, respectively,
were observed in immature bone marrow granulocytes. At this time the
incorporation of 14C- ornithine into putrescine and spermidine in bone
marrow granulocytes from stimulated animals was 14 times that of cells from
controls. A maximum increase in DNA synthesis in these cells during the
inflammatory response occurred 6 hr after the maximum increase in the
polyamine synthetic activity. Together these data suggest that polyamine
synthesis in the granulocyte compartment of the bone marrow is associated
chiefly with immature proliferating cells and that increased polyamine
synthesis precedes increased granulocyte proliferation in the bone marrow
following an inflammatory stimulus.
Volume 51,
Issue 6,
pp. 1021-1029,
06/01/1978
Copyright © 1978 by The American Society of Hematology
